ProteoCool Pills: Index

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Pils#	Title
1	Overload of centrifugal Ultra-filtration device may cause partial sample lost
2	Homemade Bradford reagent 5X
3	Homemade E.coli chemically competent cells
4	Replacement of Ethidium bromide with a safe stain
5	Safe Comassie blue staining solutions (compatible with microwave coloration)
6	Rapid protein Expression and Solubility check using a 24 tip-horn sonicator
7	Small scale IMAC purification from Expi293 or Expi-CHO culture surnatant
8	ExpiCHO a powerful cell line for transient expression of biologics
9	Use of low purity imidazole in buffer for IMAC may prevent protein detection during elution steps
10	Use of 2F-Peracetyl-Fucose to produce low fucosilated monoclonal antibodies with Expi293 and ExpiCHO cell lines
11	Chemical_enzymatic cell lysis as alternative to physical lysis in high-throughput small scale protein purification from E.coli
12	Rapid insertion/deletion/replacement of N-terminal signal peptide sequences using PIPE cloning
13	Densitometric Protein quantification from SDS-page using the ImageJ free software package
14	Tips for cell propagation and recombinant protein expression using ExpiCHO and Expi293 (WT and GNTI-)
15	An alternative way to fix the sticky pad to the Eppendorf S41i CO2 shakers
16	Loading small sample volumes on AKTA instruments
17	Alternative cheaper trasfection agents for recombinant protein/antibody expression with Expi293
18	MabSelect@ a cheaper alternative to proteinA sepharose FF resin for small scale gravity flow purification of recombinant mab
19	Pay attention to reverse GOI insertion in TOPO cloning
20	Micro PEG solubility screening, a simple Tool for Biologics Design and Formulation Development
21	Chromas a free tool to check your Sanger sequencing results
22	Tips for cleaning the OMNISEC RALS/LALS flow cell if get dirty  after the passage of biological samples
23	Selection of the right post-column filter  is essential to detect protein/antibody aggregates in static light scattering
24	Rapid fluorimetric DNA plasmid quantification on 96 welll plate
25	Choice of the right material is essential to perform DNA and/or protein UV Spectrophotometric quantifications
26	Guidelines to download a Video from blogger
27	DSF an useful tool for Monoclonal antibodies selection, optimization and developabiltiy assesment

 

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