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- Overload of centrifugal Ultra-filtration device may cause partial sample lost
- Homemade Bradford reagent 5X
- Homemade E.coli chemically competent cells
- Replacement of Ethidium bromide with a safe stain for agarose gel
- Safe Comassie blue staining for SDS page (compatible with microwave coloration)
- Rapid protein Expression and Solubility check using a 24 tip-horn sonicator
- Small scale IMAC purification from Expi293 or Expi-CHO culture surnatant
- ExpiCHO a powerful cell line for transient expression of biologics
- Use of low purity imidazole in buffer for IMAC may prevent protein detection during elution steps
- Use of 2F-Peracetyl-Fucose to produce low fucosilated monoclonal antibodies with Expi293 and ExpiCHO cell lines
- Chemical_enzymatic Cell lysis as alternative to physical lysis in high-throughput small scale protein purification from E.coli
- Rapid insertion/deletion/replacement of N-terminal signal peptide sequences using PIPE cloning
- Densitometric Protein quantification from SDS-page using the ImageJ free software package
- Propagation and recombinant protein expression using ExpiCHO and Expi293 (WT and GNTI-)
- An alternative way to fix the sticky pad to the Eppendorf S41i CO2 shakers
- Loading small sample volumes on AKTA instruments
- Alternative cheaper trasfection agents for recombinant protein/antibody expression with Expi293
- MabSelect@ a cheaper alternative to proteinA sepharose FF resin for small scale gravity flow purification of recombinant mab
- Pay attention to reverse GOI insertion in TOPO cloning
- Micro PEG solubility screening, a simple Tool for Biologics Design and Formulation Development
- Chromas a free tool to check your Sanger sequencing results
- Selection of the right post-column filter is essential to detect protein/antibody aggregates in static light scattering
- Rapid fluorimetric DNA plasmid quantification on 96 welll plate
- Choice of the right material is essential to perform DNA and/or protein UV Spectrophotometric quantifications
- Guidelines to download a Video from blogger
- DSF an useful tool for Monoclonal antibodies selection, optimization and developabiltiy assesmen
- Extracellular vesicle production using ExpiCHO
- Simple solutions to improve laboratory routine work with few euro/dollars: #1 IKEA
- How improve Specific heavy-light chain pairing in the production of bispecific mabs
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