I'm Manuele Martinelli, a chemist with almost 10 years of experience as a senior R&D scientist (focused on protein production and characterization). During my time in the lab I've had the opportunity to test a lot of instruments, procedures and reagents, and I'd like to share some Cool Protein ProtoCols (ProteCool!)with you in the hope that they help you with your experiments.Since I was born strabic, I always had a different point of view, which I want to share with you! :-)
Wednesday, May 15, 2019
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ProteoCool Pills #33: What about specificity of antibody isotyping kits?
Humans and mice share five primary antibody (immunoglobulin) isotypes: IgM, IgD, IgG, IgA, and IgE. While they share identical foundationa...
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Although imidazole itself should not give a UV280 signal, many commercially available products contain impurities which can give a strong ... -
Several different methods are currently available to perform quantification of purified recombinant proteins and antibodies There is not ... -
AKTA instruments (as AKTA-FPLC, AKTA-PRIME, AKTA-Purifiers, AKTA-Go, AKTA-pure) represent in the last 30 years the point of reference for pr...