ProteoCool Pills#1; Overload of centrifugal Ultra-filtration device may cause partial sample lost

                               

TIPS of the Week #1

(11/04/2021)

Overload of centrifugal Ultra-filtration device may cause partial sample lost

The centrifugal Ultrafiltration concentrators present in the upper part a small channel

 that allows air to enter and permit sample concentration. 

Sample

Without this channel the sample would quickly go under vacuum and the concentration would stop immediately.  

In case of sample overload, especially using swinging bucket rotors, the sample excess can leak through the channel and some sample can be lost

Conclusion: 

PAY ATTENTION TO NOT OVERFILL THE CONCENTRATION DEVICE

ProteoCool n°34: Inappropriate use of Ultrafiltration for concentration and/or buffer exchange of membrane proteins

 

                                             full video is available at PAGE4

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