ProteoCool Pills#4: Replacement of Ethidium bromide with a safe stain

 

Ethidium bromide thanks to its ability to show an intense fluorescence (ex 300nm/em 620nm) after binding with DNA.

 Ethidium bromide (EB) is inexpensive, sufficiently sensitive and very stable and thanks to this property has been the stain of choice for nucleic acid gel staining for decades.

 However on the other hands, EB is also a known powerful mutagen.

Recent years alternative reagents able to show an intense fluorescence after binding with DNA but not able to permeate cell membrane were developed:

 

My preferred one:

 

Gel REd - Biotium  (VWR cod. 41003) 

   https://biotium.com/product/gelred-nucleic-acid-gel-stain/

 

Safety assessment available at:

 

https://biotium.com/wp-content/uploads/2013/07/GelRed-and-GelGreen-Safety-Report.pdf

 

Cost: About 90€ for 500ul (stock 10000X) à  90€ for 5liter of agarose à 0,9€ for a mini gel (15x2well)

                                                                                                                                  2,5€ for a midi gel (up to 30x2 well)

                                                                                                                                     

Another alternative that I used in the past is the Sybr Safe (thermo cod. S33102) 

https://www.thermofisher.com/order/catalog/product/S33102?SID=srch-srp-S33102#/S33102?SID=srch-srp-S33102 

is another possible alternative. but in my experience, it shows limited stable under UV excitation.

 

More details are available in the following link

 

https://www.blogger.com/video.g?token=AD6v5dxB5wweSgHEks3TU3bm1QoGEYZU6GT8788soF0i7SEVvaQwM2AgHXmDY62fIzukFrMMDbFQEFOPMfSyV4GwvJHPY9U__KiUhmaCsx6NAwneL2yJYbaSu_DV-H4_to6BznMtlE59

Buon Compleanno ProteoCool!

 

Happy birthday ProteoCool!

https://proteocool.blogspot.com/

Sono già 2 anni da quando ho avuto la folle idea di provare a dedicare parte delle mie serate lombarde alla creazione di un semplice #blog #scientifico per condividere le mie conoscenze con chi muove i primi passi nell'affascinante mondo delle proteine

E' una cosa semplice, artigianale e con una grafica un po anni 80: un mio amico ha detto che sembra un gioco dell'ATARI , ma spero che anche se la forma può essere migliorata, il contenuto sia utile a qualcuno!

ProteoCool è ancora un bimbo che ha mosso i primi passi ma che ha bisogno del supporto di tutti per crescere.

Se vi va condividetelo!! Grazie in anticipo! Manu!
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2 years ago, i had the crazy idea to spend my evenings to build a simple scientific blog to share some pills with newbies in the fascinating field of protein science.

ProteoCool is a simple and handmade blog.

ProteoCool is still a baby and he need your support to growth.

Please, if you like it, share its address with your network!

Thanks in advance. Manu!

ProteoCool n°27 Overview on AGAROSE-GEL

 

 

is now available at PAGE1 of ProteoCool