ProteoCool n°30
Uniform 15N, 13C and 2H protein labelling using E.coli and more
I'm Manuele Martinelli, a chemist with almost 10 years of experience as a senior R&D scientist (focused on protein production and characterization). During my time in the lab I've had the opportunity to test a lot of instruments, procedures and reagents, and I'd like to share some Cool Protein ProtoCols (ProteCool!)with you in the hope that they help you with your experiments.Since I was born strabic, I always had a different point of view, which I want to share with you! :-)
Showing posts with label 15N. Show all posts
Showing posts with label 15N. Show all posts
Saturday, May 23, 2020
ProteoCool n°30 Uniform 15N, 13C and 2H protein labelling using E.coli and more
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For many years the application of the traditional adherent mammalian cell cultures for recombinant protein production was difficult and appl...
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Several different methods are currently available to perform quantification of purified recombinant proteins and antibodies There is not ... -
Although imidazole itself should not give a UV280 signal, many commercially available products contain impurities which can give a strong ...
