ProteoCool n°30
Uniform 15N, 13C and 2H protein labelling using E.coli and more
I'm Manuele Martinelli, a chemist with almost 10 years of experience as a senior R&D scientist (focused on protein production and characterization). During my time in the lab I've had the opportunity to test a lot of instruments, procedures and reagents, and I'd like to share some Cool Protein ProtoCols (ProteCool!)with you in the hope that they help you with your experiments.Since I was born strabic, I always had a different point of view, which I want to share with you! :-)
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ProteoCool Pills #32: HemA, a powerfull selection marker for antibiotic free plasmid mantainance for recombinant protein expression in E.coli
Antibiotic resistance genes (e.g Ampicillin , Kanamycin ) are the most commonly used markers for plamisd selection in DNA production and r...
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Although imidazole itself should not give a UV280 signal, many commercially available products contain impurities which can give a strong ... -
Several different methods are currently available to perform quantification of purified recombinant proteins and antibodies There is not ... -
In both eukaryotic and prokaryotic cells, all proteins are synthesized in cytoplasm. Proteins that are destined to enter into the secreto...
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