ProteoCool Pills#2: Homemade Bradford reagent 5X

 

TIPS of the Week #2

(18/04/2021)

Homemade Bradford reagent 5X 

(for 1 liter)

100mg Comassie Brilliant Blue G-250

dissolved in

-         48 ml of absolute EtOH

-         100 ml 85% (w/v) H₃PO₄

 

Mix well until the die is completely dissolved

Dilute up to 1liter with milliQ water and filter through a 0.45uM PDVF filtrer.

Store the dye at 4°C and use as 5X solution.

 

Cost for 1 liter:

-         Comassie G250 (Thermo cod. 20279 cost 144€/50g à 0,3€

-         EtOH (Sigma Aldrich cod. 51976 cost: 100€/500ml ~ 10€

-         H₃PO₄ (eg FisherScientific cod. 13207659 cost 48,2€/500ml ~ 4.8€

 

Total <20€ for 1 liter that is very low if you consider that

Biorad Protein Assay reagent 5X cod. 5000006 à 180€ for 500ml