ProteoCool n°30
Uniform 15N, 13C and 2H protein labelling using E.coli and more
I'm Manuele Martinelli, a chemist with almost 10 years of experience as a senior R&D scientist (focused on protein production and characterization). During my time in the lab I've had the opportunity to test a lot of instruments, procedures and reagents, and I'd like to share some Cool Protein ProtoCols (ProteCool!)with you in the hope that they help you with your experiments.Since I was born strabic, I always had a different point of view, which I want to share with you! :-)
Saturday, May 23, 2020
ProteoCool n°30 Uniform 15N, 13C and 2H protein labelling using E.coli and more
Tuesday, April 28, 2020
Sunday, March 22, 2020
Sunday, March 1, 2020
Monday, February 3, 2020
Saturday, January 18, 2020
Sunday, October 27, 2019
Sunday, September 15, 2019
ProteoCool n°23: TAQNOVA-RED: A cheap master mix TAQ for PCR colony screening
ProteoCool n°23
(TAQNOVA-RED: A cheap master mix TAQ for PCR colony screening)
is now available at PAGE6 of ProteoCool (https://proteocool.blogspot.com/)
#pcr #cloning #polimerase #molecularbiology #science #biotech #biology #phd #OER #academy
Monday, August 19, 2019
Monday, July 22, 2019
ProteoCool n°22: Overview on Tools for recombinant protein expression in E.coli
ProteoCool n°22:
Overview on Tools for recombinant protein expression in E.coli
is now available at PAGE 2 (https://proteocool.blogspot.com/)
#protein #science #blog #biochemistry # biology #biotech #phd
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Posts (Atom)
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For many years the application of the traditional adherent mammalian cell cultures for recombinant protein production was difficult and appl...
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Several different methods are currently available to perform quantification of purified recombinant proteins and antibodies There is not ... -
Although imidazole itself should not give a UV280 signal, many commercially available products contain impurities which can give a strong ...
