ProteoCool Pills#1; Overload of centrifugal Ultra-filtration device may cause partial sample lost

                               

TIPS of the Week #1

(11/04/2021)

Overload of centrifugal Ultra-filtration device may cause partial sample lost

The centrifugal Ultrafiltration concentrators present in the upper part a small channel

 that allows air to enter and permit sample concentration. 

Sample

Without this channel the sample would quickly go under vacuum and the concentration would stop immediately.  

In case of sample overload, especially using swinging bucket rotors, the sample excess can leak through the channel and some sample can be lost

Conclusion: 

PAY ATTENTION TO NOT OVERFILL THE CONCENTRATION DEVICE

ProteoCool N°37: Simple TIPS on useful EXCEL features for DATA handling, elaboration and representation

 ProteoCool N°37:

                  Simple TIPS on useful EXCEL features for DATA handling, elaboration and representation

                                                        is now available at PAGE7 on ProteoCool

ProteoCool n°36: 2 component Epoxy adhesive, a smart tool to ready FIX broken plastic laboratory equipment

 ProteoCool n°36

         2 component Epoxy adhesive, a smart tool to ready FIX broken plastic laboratory equipment

                                                        is now available at PAGE 7 

ProteoCool n°35: Wrong AMPICILLIN concentration for the transformation of BL21(DE3) Delta TolR E.coli

                                                              ProteoCool n°35

Wrong AMPICILLIN concentration for the transformation of BL21(DE3) Delta TolR E.coli  

is now available on #ProteoCool 

 

                               !! An updated VIDEO PLAY LIST is now available on ProteoCool !!

Click on the ProteoCool TITLE to be re-directed to the video

Proteocool n°	Title	page
1	Cloning methods overview	1
2	Primer Design: Restiction/Ligation	1
3	Primer Design: PIPE Cloning	1
4	PCR Colony screening	1
5	E.coli high cell density media overview	2
6	Rapid SDS-page coloration/decoloration using microwave	3
7	High cell density E.coli protein expression using EnpressoB media	2
8	Buffer Exchange methods overview	3
9	2 Common mistakes causing sample lost using the AKTA Systems	4
10	PARAFILM a simple support for sample loading on agarose gel	1
11	Rapid buffer <--> Exchange by PD-10	3
12	EZNA HP MINI KIT II: MIDI KIT YIELDS from a MINI KIT format	6
13	Subctractive IMAC	3
14	Endotrap: A simple and efficient tool for endotoxin removal	3
15	Overview on SDS-page	3
16	3 common mistakes in buffer preparation for protein purification	4
17	Overview on basic bioinformatic tools for DNA sequence analysis	6
18	Simple PCR test for Mycoplasma detection	5
19	Simple bioinformatic Tools for protein sequence analysis	6
20	FAST READ 102: A multi-chamber device to speed up manual cell count	6
21	3 common mistakes in E.coli culture	4
22	Overview on tools for recombinant protein expression  in E.coli	2
23	Taq Nova Red: A cheap master mix TAQ for PCR colony screening	6
24	DSF a simple and powerful tool for protein QC	3
25	A Simple way to clean and re-generate IMAC coloumns	7
26	Single channel pipette cleaning	7
27	Overview on Agarose Gel	1
28	Zinc: An alternative cheap and healthy metal for IMAC	3
29	Expi293: A simple and powerful mammalian expression system	2
30	Uniform 15N, 13C and 2H protein labelling using E.coli and more	2
31	Homemade Handrub Sanitizer	7
32	High cell density E.coli protein expression using YGR media	2
33	Simple TIPS to avoid common mistakes in Spettroptotometry/Fluorimetry/Luminometry	4
34	Inappropriate use of Ultrafiltration  for membrane protein  concentration	4

Click on PAGE number to be re-directed to the PAGE

 

 

 

 

 

ProteoCool n°34: Inappropriate use of Ultrafiltration for concentration and/or buffer exchange of membrane proteins

 

                                             full video is available at PAGE4

                                             ProteoCool: PAGE4: Common mistakes 

                                             

Buon Compleanno ProteoCool!

 

Happy birthday ProteoCool!

https://proteocool.blogspot.com/

Sono già 2 anni da quando ho avuto la folle idea di provare a dedicare parte delle mie serate lombarde alla creazione di un semplice #blog #scientifico per condividere le mie conoscenze con chi muove i primi passi nell'affascinante mondo delle proteine

E' una cosa semplice, artigianale e con una grafica un po anni 80: un mio amico ha detto che sembra un gioco dell'ATARI , ma spero che anche se la forma può essere migliorata, il contenuto sia utile a qualcuno!

ProteoCool è ancora un bimbo che ha mosso i primi passi ma che ha bisogno del supporto di tutti per crescere.

Se vi va condividetelo!! Grazie in anticipo! Manu!
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2 years ago, i had the crazy idea to spend my evenings to build a simple scientific blog to share some pills with newbies in the fascinating field of protein science.

ProteoCool is a simple and handmade blog.

ProteoCool is still a baby and he need your support to growth.

Please, if you like it, share its address with your network!

Thanks in advance. Manu!

ProteoCool n°33 Simple TIPS for avoid common mistakes in Spettrophotometry/Fluorimetry/Luminometry

                                                                  

is now available at PAGE 4

ProteoCool n°32 High Cell density protein expression in E.coli using YGR media

 

is now available on https://proteocool.blogspot.com/