ProteoCool n°35: Wrong AMPICILLIN concentration for the transformation of BL21(DE3) Delta TolR E.coli

                                                              ProteoCool n°35

Wrong AMPICILLIN concentration for the transformation of BL21(DE3) Delta TolR E.coli  

is now available on #ProteoCool 

 

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Proteocool n°	Title	page
1	Cloning methods overview	1
2	Primer Design: Restiction/Ligation	1
3	Primer Design: PIPE Cloning	1
4	PCR Colony screening	1
5	E.coli high cell density media overview	2
6	Rapid SDS-page coloration/decoloration using microwave	3
7	High cell density E.coli protein expression using EnpressoB media	2
8	Buffer Exchange methods overview	3
9	2 Common mistakes causing sample lost using the AKTA Systems	4
10	PARAFILM a simple support for sample loading on agarose gel	1
11	Rapid buffer <--> Exchange by PD-10	3
12	EZNA HP MINI KIT II: MIDI KIT YIELDS from a MINI KIT format	6
13	Subctractive IMAC	3
14	Endotrap: A simple and efficient tool for endotoxin removal	3
15	Overview on SDS-page	3
16	3 common mistakes in buffer preparation for protein purification	4
17	Overview on basic bioinformatic tools for DNA sequence analysis	6
18	Simple PCR test for Mycoplasma detection	5
19	Simple bioinformatic Tools for protein sequence analysis	6
20	FAST READ 102: A multi-chamber device to speed up manual cell count	6
21	3 common mistakes in E.coli culture	4
22	Overview on tools for recombinant protein expression  in E.coli	2
23	Taq Nova Red: A cheap master mix TAQ for PCR colony screening	6
24	DSF a simple and powerful tool for protein QC	3
25	A Simple way to clean and re-generate IMAC coloumns	7
26	Single channel pipette cleaning	7
27	Overview on Agarose Gel	1
28	Zinc: An alternative cheap and healthy metal for IMAC	3
29	Expi293: A simple and powerful mammalian expression system	2
30	Uniform 15N, 13C and 2H protein labelling using E.coli and more	2
31	Homemade Handrub Sanitizer	7
32	High cell density E.coli protein expression using YGR media	2
33	Simple TIPS to avoid common mistakes in Spettroptotometry/Fluorimetry/Luminometry	4
34	Inappropriate use of Ultrafiltration  for membrane protein  concentration	4

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ProteoCool n°34: Inappropriate use of Ultrafiltration for concentration and/or buffer exchange of membrane proteins

 

                                             full video is available at PAGE4

                                             ProteoCool: PAGE4: Common mistakes