Sunday, December 13, 2020

 

                               !! An updated VIDEO PLAY LIST is now available on ProteoCool !!

Click on the ProteoCool TITLE to be re-directed to the video

Proteocool n°

Title

page

1

Cloning methods overview

1

2

Primer Design: Restiction/Ligation

1

3

Primer Design: PIPE Cloning

1

4

PCR Colony screening

1

5

 E.coli high cell density media overview

2

6

Rapid SDS-page coloration/decoloration using microwave

3

7

High cell density E.coli protein expression using EnpressoB media 

2

8

Buffer Exchange methods overview

3

9

2 Common mistakes causing sample lost using the AKTA Systems

4

10

PARAFILM a simple support for sample loading on agarose gel

1

11

Rapid buffer <--> Exchange by PD-10

3

12

EZNA HP MINI KIT II: MIDI KIT YIELDS from a MINI KIT format

6

13

Subctractive IMAC

3

14

Endotrap: A simple and efficient tool for endotoxin removal

3

15

Overview on SDS-page

3

16

3 common mistakes in buffer preparation for protein purification

4

17

Overview on basic bioinformatic tools for DNA sequence analysis

6

18

Simple PCR test for Mycoplasma detection

5

19

Simple bioinformatic Tools for protein sequence analysis

6

20

FAST READ 102: A multi-chamber device to speed up manual cell count

6

21

3 common mistakes in E.coli culture

4

22

Overview on tools for recombinant protein expression  in E.coli

2

23

Taq Nova Red: A cheap master mix TAQ for PCR colony screening 

6

24

DSF a simple and powerful tool for protein QC

3

25

Simple way to clean and re-generate IMAC coloumns 

7

26

Single channel pipette cleaning

7

27

Overview on Agarose Gel

1

28

Zinc: An alternative cheap and healthy metal for IMAC

3

29

Expi293: A simple and powerful mammalian expression system

2

30

Uniform 15N13C and 2H protein labelling using E.coli and more

2

31

Homemade Handrub Sanitizer

7

32

High cell density E.coli protein expression using YGR media 

2

33

Simple TIPS to avoid common mistakes in Spettroptotometry/Fluorimetry/Luminometry

4

34

Inappropriate use of Ultrafiltration  for membrane protein  concentration

4

Click on PAGE number to be re-directed to the PAGE

 
 
 
 
 

Tuesday, December 1, 2020

ProteoCool n°34: Inappropriate use of Ultrafiltration for concentration and/or buffer exchange of membrane proteins

 

                                             full video is available at PAGE4

                                             ProteoCool: PAGE4: Common mistakes 

                                             

usefull links #1

i would like with share the folliwng 3 links about usefull on line tool for the scientist working with recombinant monoclonal antibodies:   ...